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NATURAL BIOMARKERS FOR CELLULAR METABOLISM: BIOLOGY TECHNIQUES AND APPLICATIONS (pb) 2018
Author: VLADIMIR V. GHUKASYAN
ISBN: 9781138198784
Year: 2018
Publisher: CRC PRESS
Category: PHYSICS
Edition: 1
Format: Paperback
Language: English
Pages: 408
 ABOUT THE TITLE
Features
•Provides the biological background associated with energy metabolism and other metabolic pathways as well as the spectroscopy of potential biomarkers intrinsic in living cells and tissues
•Explores recent advances in natural biomarkers, including quantitative, noninvasive optical methods for cellular/tissue metabolism studies


• Covers the theoretical and technical aspects of various fluorescence microscopy and spectroscopy methods used to monitor the autofluorescence of natural biomarkers in living cells/tissues

• Explains how to use cellular autofluorescence to investigate metabolic activities, mitochondrial anomalies, and biochemical redox reactions in healthy cells/tissues under different external manipulations induced by drugs, diseases, or environmental hazards

• Describes the latest laser-based medical diagnostic approaches that address the cellular and biochemical mechanisms of health problems in both quantitative and noninvasive ways

Summary
From the Lab to Clinical Settings—
Advances in Quantitative, Noninvasive Optical Diagnostics


Noninvasive fluorescence imaging techniques, novel fluorescent labels, and natural biomarkers are revolutionizing our knowledge of cellular processes, signaling and metabolic pathways, the underlying mechanisms for health problems, and the identification of new therapeutic targets for drug discoveries. Natural Biomarkers for Cellular Metabolism: Biology, Techniques, and Applications delves into the current state of knowledge on intrinsic fluorescent biomarkers and highlights recent developments in using these biomarkers for the metabolic mapping and clinical diagnosis of healthy and diseased cells and tissues.

Autofluorescent Biomarkers for Biomedical Diagnostics

The book’s first section introduces the fundamentals of cellular energy metabolism as well as natural biomarkers within the context of their biological functions. The second section outlines the theoretical and technical background of quantitative, noninvasive, autofluorescence microscopy and spectroscopy methods, including experimental design, calibration, pitfalls, and remedies of data acquisition and analysis. The last two sections highlight advances in biomedical and biochemical applications, such as monitoring stem cell differentiation in engineered tissues and diagnosing cancer and ophthalmic diseases quantitatively and noninvasively.

Tailored to Interdisciplinary Researchers

Covering cell biology, imaging techniques, and clinical diagnostics, this book provides readers with a complete guide to studying cellular/tissue metabolism under healthy, diseased, and environment-induced stress conditions using natural biomarkers. The book is designed for graduate and advanced undergraduate students, biophysics instructors, medical researchers, and those in pharmaceutical R&D.



Editor: Vladimir V. Ghukasyan is a research assistant professor in the Department of Cell Biology and Physiology and a director of the Confocal and Multiphoton Imaging Facility of the Neuroscience Center at the University of North Carolina at Chapel Hill. He earned a PhD in biology from the Institute of Biotechnology, Yerevan, Armenia, and completed postdoctoral training at the Institute of Biophotonics, Taipei, Taiwan.


Ahmed A. Heikal is a professor in the Department of Chemistry and Biochemistry, Swenson College of Science and Engineering at the University of Minnesota Duluth. His research interests in molecular and cellular biophysics were inspired by his work with Watt W. Webb as a postdoctoral associate at Cornell University. Dr. Heikal earned a PhD in applied physics from the California Institute of Technology under the supervision of Nobel Laureate Ahmed H. Zewail.
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 TABLE OF CONTENTS
Biochemical, Biological, and Biophysical Background
Mitochondria and Energy Metabolism: Networks, Mechanisms, and Control Ilmo E. Hassinen
Intracellular Autof luorescent Species: Structure, Spectroscopy, and Photophysics Nobuhiro Ohta and Takakazu Nakabayashi
Autofluorescence Imaging Techniques: Fundamentals and Applications
One-Photon Autof luorescence Microscopy Narasimhan Rajaram and Nirmala Ramanujam
Autofluorescence Lifetime Imaging Michael G. Nichols, Kristina Ward, Lyandysha V. Zholudeva, Heather Jensen Smith, and Richard Hallworth
Polarization Imaging of Cellular Autofluorescence Harshad D. Vishwasrao, Qianru Yu, Kuravi Hewawasam, and Ahmed A. Heikal
Real-Time In Vivo Monitoring of Cellular Energy Metabolism Avraham Mayevsky and Efrat Barbiro-Michaely
Tryptophan as an Alternative Biomarker for Cellular Energy Metabolism Vinod Jyothikumar, Yuansheng Sun, and Ammasi Periasamy
Alternative Approaches to Optical Sensing of the Redox State Yi Yang
Natural Biomarkers for Biochemical and Biological Studies
Spatiotemporal Detection of NADH-Linked Enzyme Activities in Single Cell Metabolism V. Krishnan Ramanujan
NAD(P)H and FAD as Biomarkers for Programmed Cell Death Hsing-Wen Wang
Monitoring Stem Cell Differentiation in Engineered Tissues Kyle P. Quinn and Irene Georgakoudi
Autofluorescence as a Diagnostic Tool in Medicine and Health
Autofluorescence-Assisted Examination of Cardiovascular System Physiology and Pathology Alzbeta Marcek Chorvatova
Autofluorescence Perspective of Cancer Diagnostics Lin Z. Li and Nannan Sun
Dynamic Imaging of Intracellular Coenzyme Autofluorescence in Intact Pancreatic Islets Alan K. Lam and Jonathan V. Rocheleau
Autofluorescence Diagnostics of Ophthalmic Diseases Dietrich Schweitzer
Pathogen Effects on Energy Metabolism in Host Cells Márta Szaszák and Jan Rupp
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